Basic information
PhaSepDB ID mloht2199 Uniprot Entry P35637 Gene names FUS
Class MLO-ht MLO Nucleolus Organism Homo sapiens
Cell line HeLa cells Method organelle purification PMID 15635413
Publication note HeLa cell nucleoli were isolated in high purity by density gradient fractionation. Using mass-spectrometry-based organellar proteomics and stable isotope labelling, we perform a quantitative analysis of the proteome of human nucleoli. In vivo fluorescent imaging techniques are directly compared to endogenous protein changes measured by proteomics.