PhaSepDB2.1 : The database of phase-separation related proteins

PhaSepDB provides a collection of manually curated phase separation (PS) proteins and Membraneless organelles (MLOs) related proteins. As of June 2022, 1419 PS entries, 507 high throughput PS entries73 MLOs, 770 low throughput MLO related entries and 7303 high throughput MLO related entries were included.
PS entries were classified into 511 “PS-self” (the protein can undergo PS in vitro by itself) and 908 “PS-other” entries (the protein require other partners to form biomolecular droplet in vitro or can form or be recruited to biomolecular droplet in vivo). We provide detailed annotation for PS entries, including the material states of the PS droplets, PS verification experiments, the regions used in experiments, phase diagram, PS partners (protein, RNA and others), and the regulation (mutation, modification, etc.) of the proteins' PS ability.


Examples : psself204, NSP5, P54274, Rattus norvegicus

Predict phase separation proteins, search phase separation and MLO related diseases.


Browse all phase separation entries in PhaSepDB by combinatorial search.

Browse MLOs

Explore all MLOs, you can see the description and related proteins for each MLO.

2023-06-15: High throughput phase separation protein from AICAP was updated to PhaSepDB2.1,click here for more detail.
2022-09-17: PhaSepDB2.1 was published on Nucleic Acids Research.
2022-06-17: PhaSepDB2.1 was online.
2021-07-13: PhaSepDB2.0 was online.
2019-10-04: PhaSepDB1.0 was published on Nucleic Acids Research.

Chao Hou, Xinxin Wang, Haotai Xie, Taoyu Chen, Peiyu Zhu, Xiaofeng Xu, Kaiqiang You, Tingting Li. PhaSepDB in 2022: annotating phase separation-related proteins with droplet states, co-phase separation partners and other experimental information. Nucleic Acids Research