Basic information
LLPS related evidences
| Publication note | The core active-zone scaffold proteins SYD-2 (also known as liprin-α) and ELKS-1 undergo phase separation during an early stage of synapse development, and later mature into a solid structure. We directly test the in vivo function of phase separation by using mutant SYD-2 and ELKS-1 proteins that specifically lack this activity. These mutant proteins remain enriched at synapses in Caenorhabditis elegans, but show defects in active-zone assembly and synapse function. The defects are rescued by introducing a phase-separation motif from an unrelated protein. In vitro, we reconstitute the SYD-2 and ELKS-1 liquid-phase scaffold, and find that it is competent to bind and incorporate downstream active-zone components. We find that the fluidity of SYD-2 and ELKS-1 condensates is essential for efficient mixing and incorporation of active-zone components. (Organism: Caenorhabditis elegans; Cell line: C.elegans cells) |
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| Material state | liquid,solid : SYD-2 (also known as liprin-α) and ELKS-1 undergo phase separation during an early stage of synapse development, and later mature into a solid structure. | ||
| LLPS region | 300-449,716-836,716-775,807-836,262-336,300-375,262-375,1-836,1-300+336-746+776-836,1-300+336-715+776-806 | Key domains | _ : _ |
LLPS partner
| Protein partner | _ ( _ ) |
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|---|---|---|
| RNA partner | _ |
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| Others | _ |
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LLPS regulation
| PTM | _ |
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|---|---|---|
| Mutation | del747-775 |
Small targeted deletions in these motifs blocked LLPS in full-length ELKS-1. |
| Alternative splicing | _ | |
| Repeat | _ | |
| Oligomerization | _ | |