Basic information
LLPS related evidences
| Publication note | To investigate whether a physical interaction exists between cGAS-DNA complexes and TREX1 in cells, we transfected cells with Cy3-labeled plasmid DNA and tracked the localization of cGAS and TREX1. Following DNA transfection, endogenous cGAS forms puncta that co-localize with Cy3-labeled DNA and are consistent with observations made previously in a variety of cell types. In agreement with our hypothesis, GFP-TREX1 also co-localizes with cGAS-DNA puncta in the cytosol, suggesting active interaction between the cGAS-DNA complex and negative regulators of DNA immune sensing. (Organism: Homo sapiens; Cell line: MCF10A cells) |
||
|---|---|---|---|
| Material state | liquid : TREX1 alone can undergo a phase separation transition in vitro in the presence of dsDNA to form dynamic liquid-like droplets | ||
| LLPS region | 1-313 | Key domains | _ : _ |
LLPS partner
| Protein partner | hcGAS ( Q8N884 ) |
GFP-TREX1 also co-localizes with cGAS-DNA puncta in the cytosol |
|---|---|---|
| RNA partner | _ |
_ |
| Others | ssDNA,dsDNA |
TREX1 is able to form liquid droplets in the presence of ssDNA. TREX1 alone can undergo a phase separation transition in vitro in the presence of dsDNA to form dynamic liquid-like droplets. |
LLPS regulation
| PTM | _ |
_ |
|---|---|---|
| Mutation | p.E198K |
TREX1 E198K requires a lower protein concentration to begin interaction with the cGAS-DNA droplet periphery, and TREX1 E198K readily permeates into the internal suppressive environment at the droplet center. |
| Alternative splicing | _ | |
| Repeat | _ | |
| Oligomerization | _ | |