Basic information
PhaSepDB ID psother409 Reference 32881044,29507397 Uniprot Entry Q13501 Gene names p62
Class PS-other Location Cytoplasm MLO p62 cluster
LLPS related evidences
Publication note Here we report that p62 forms droplets in vivo which have liquid-like properties such as high sphericity, the ability to undergo fusion, and recovery after photobleaching. Recombinant p62 does not undergo phase separation in vitro; however, adding a K63 polyubiquitin chain to p62 induces p62 phase separation, which results in enrichment of high-molecular weight ubiquitin signals in p62 droplets. Mixing recombinant p62 with cytosol from p62-/- cells also results in p62 phase separation in a polyubiquitination-dependent manner.
(Organism: Homo sapiens; Cell line: NRK cells)
Material state liquid : P62 bodies have viscous liquid-like properties.
LLPS region 3-440 Key domains PB1 domain,UBA domain : The PB1 domain and UBA domain of p62 are required for polyubiquitin chain-induced phase separation and autophagic degradation of p62
LLPS partner
Protein partner

K63 polyubiquitin chain ( _ )

 Recombinant p62 does not undergo phase separation in vitro; however, adding a K63 polyubiquitin chain to p62 induces p62 phase separation,
RNA partner

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Others

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LLPS regulation
PTM

S403;phosphorylation

Phosphorylation of S403 promotes polyubiquitin chain-induced phase separation, p62 body formation, and autophagic degradation
Mutation

p.S403E,p.M404T,p.G411S

p62-S403E formed significantly larger bodies than wild-type p62. We generated p62-GFP with two Paget’s disease of bone (PDB) mutations, M404T and G411S. p62 mutations identified in PDB affect p62 body formation, autophagic degradation of p62, and p62 phase separation
Alternative splicing _
Repeat _
Oligomerization _