Basic information
PhaSepDB ID psother353 Reference 32385244 Uniprot Entry Q9JK83 Gene names Par6
Class PS-other Location Cytoplasm MLO Par complex
LLPS related evidences
Publication note Here we show that the Par complex exhibits cell cycle-dependent condensation in Drosophila neuroblasts, driven by liquid-liquid phase separation. The open conformation of Par3 undergoes autonomous phase separation likely due to its NTD-mediated oligomerization. Par6, via C-terminal tail binding to Par3 PDZ3, can be enriched to Par3 condensates and in return dramatically promote Par3 phase separation. aPKC can also be concentrated to the Par3N/Par6 condensates as a client. Interestingly, activated aPKC can disperse the Par3/Par6 condensates via phosphorylation of Par3. Perturbations of Par3/Par6 phase separation impair the establishment of apical-basal polarity during neuroblast asymmetric divisions and lead to defective lineage development.
(Organism: Mus musculus; Cell line: COS-7 cells)
Material state liquid,hydrogel : Those Par3N/Par6β droplets transitioned into gel-like structures over time, and the droplets rarely fused together after 20 min.
LLPS region 1-371 Key domains PDZ-binding motif (PBM) : Par3N specifically binds to Par6β via the PDZ3–PBM recognition.
LLPS partner
Protein partner

Par3,aPKC ( Q9Z340,Q62074 )

 Par6, via C-terminal tail binding to Par3 PDZ3, can be enriched to Par3 condensates and in return dramatically promote Par3 phase separation.
RNA partner

_

 _
Others

_

 _
LLPS regulation
PTM

_

_
Mutation

_

_
Alternative splicing _
Repeat _
Oligomerization _