Basic information
LLPS related evidences
| Publication note | Here, we report that the replication initiation machinery of D. melanogaster unexpectedly undergoes liquid-liquid phase separation (LLPS) upon binding DNA in vitro. We find that ORC, Cdc6, and Cdt1 contain intrinsically disordered regions (IDRs) that drive LLPS and constitute a new class of phase separating elements. Initiator IDRs are shown to regulate multiple functions, including chromosome recruitment, initiator-specific co-assembly, and Mcm2-7 loading. These data help explain how CDK activity controls replication initiation and suggest that replication programs are subject to higher-order levels of inter-origin organization. (Organism: Drosophila melanogaster; Cell line: _) |
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| Material state | liquid : Biochemical studies show that these IDRs drive the selective co-assembly and enrichment of Drosophila initiation factors into liquid phases in the presence of DNA, while simultaneously excluding non-partner proteins that also phase separate. | ||
| LLPS region | 1-257 | Key domains | _ : _ |
LLPS partner
| Protein partner | ORC1,ORC1,ORC2,ORC3,ORC4,ORC5 ( O16810,Q24168,Q7K2L1,Q9W102,Q24169 ) |
ORC consists of a heterohexameric complex comprising the subunits Orc1-6. Drosophila ORC phase separates using interactions that require the N-terminal IDR of Orc1 but not Orc2. |
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| RNA partner | _ |
_ |
| Others | dsDNA |
like Cdt1, ORC is able to undergo LLPS at physiological concentrations in the presence of a variety of DNA substrates without any apparent DNA sequence dependence |
LLPS regulation
| PTM | _ |
_ |
|---|---|---|
| Mutation | _ |
_ |
| Alternative splicing | _ | |
| Repeat | _ | |
| Oligomerization | _ | |