Basic information
LLPS related evidences
| Publication note | We show that DNA sequences encoding TF binding site number, density, and affinity above sharply defined thresholds drive condensation of TFs and coactivators. A combination of specific structured (TF-DNA) and weak multivalent (TF-coactivator) interactions allows for condensates to form at particular genomic loci determined by the DNA sequence and the complement of expressed TFs. DNA features found to drive condensation promote enhancer activity and transcription in cells. For TF and coactivator, we employed purified OCT4, a master transcription factor in murine embryonic stem cells (mESCs), and MED1-IDR, the intrinsically disordered region of the largest subunit of the Mediator coactivator complex. (Organism: Mus musculus; Cell line: _) |
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|---|---|---|---|
| Material state | _ : _ | ||
| LLPS region | IDR region | Key domains | intrinsically disordered region (IDR) : ranscriptional condensate stability is governed by a combination of TF-DNA and IDR-IDR interactions between TFs and coactivators(like MED1) |
LLPS partner
| Protein partner | OCT4 ( P20263 ) |
in vitro assays revealed that ODNA_20 promoted phase separation of MED1-IDR and OCT4 at lower concentrations |
|---|---|---|
| RNA partner | _ |
_ |
| Others | ODNA_20 |
in vitro assays revealed that ODNA_20 promoted phase separation of MED1-IDR and OCT4 at lower concentrations |
LLPS regulation
| PTM | _ |
_ |
|---|---|---|
| Mutation | _ |
_ |
| Alternative splicing | _ | |
| Repeat | _ | |
| Oligomerization | _ | |