Basic information
PhaSepDB ID psother126 Reference 30173914 Uniprot Entry G5EC37 Gene names SEPA-1
Class PS-other Location Nucleus MLO P granule
LLPS related evidences
Publication note Here, we demonstrated that autophagic degradation of PGL granules is specified by phase separation and transition that are controlled by SEPA-1, EPG-2, and PTMs. SEPA-1 promotes LLPS of PGL-1 and PGL-3. EPG-2 determines the size of droplets and also converts them into gel-like structures.
(Organism: Caenorhabditis elegans; Cell line: C.elegans embryos)
Material state liquid,hydrogel : EPG-2/SEPA-1/PGL-1/-3 liquid-like droplets develop gel-like characteristics over time, including impaired fusion capability, increased resistance to high salt, and reduced dynamics.
LLPS region 1-350,1-702 Key domains PGL-3-binding domain : The region in SEPA-1 that promotes LLPS of PGL-1/-3 was delineated to the N-terminal region, which contains the PGL-3-binding domain.
LLPS partner
Protein partner

PGL-1,PGL-3 ( Q9TZQ3,G5EBV6 )

 Purified SEPA-1 failed to phase separate. Co-addition of SEPA-1 promoted LLPS of PGL-3 and PGL-1/-3, but not PGL-1 alone. In the presence of SEPA-1, larger droplets formed and more PGL-1/-3 proteins partitioned into droplets.
RNA partner

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Others

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LLPS regulation
PTM

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Mutation

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Alternative splicing _
Repeat _
Oligomerization _